Evaluation of Inhibitory Effects of Actinomycetes Agains Urinary Tract Infection Bacteria

Leong, Chiau Wei (2018) Evaluation of Inhibitory Effects of Actinomycetes Agains Urinary Tract Infection Bacteria. Final Year Project (Bachelor), Tunku Abdul Rahman University College.

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Abstract

The discovery of actinomycetes inhibits on urinary treat infection (UTI) disease. Actinomycetes are gram positive bacteria with high G+C content and produce antimicrobial compounds under certain conditions and growth media. This microbial group has also contributed to the discovery of antibiotics. UTI is a bacterial infection causing painful and annoying symptoms which could lead to serious consequences. Although treatable with antibiotics, antibiotic resistances have reduced the available options to control this infection. Thus, the objectives of this study were to isolate the actinomycetes from the banana soil with potentials to suppress UTI bacteria and determined the antibacterial activity of actinomycetes against UTI bacteria. Soil samples were obtained from drip zone of a banana tree at Mentakab, Pahang. Isolation of the actinomycetes was performed by using the selective agar medium known as actinomycetes isolation agar (AIA). The actinomycetes colonies that appeared on the AIA were isolated and group according to physical characteristics. Dual culture challenge tests were performed to determine the inhibitory effects towards UTI bacteria measured by percent inhibition of diameter growth (PIDG). There were a total of 13 types of actinomycetes have been successfully isolated from the soil. The isolated actinomycetes were then subjected to antagonistic tests against the UTI bacteria available in the laboratory i.e. Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and Pseudomonas aeruginosa. The antagonistic tests were performed using agar diffusion test. Among 13 actinomycetes tested against the above mentioned UTI bacteria, 7 of them showed various degrees of inhibitory effects. The inhibitory results ranged from 30 % to 75%. Identification of the seven actinomycetes were performed via 16s rDNA gene sequencing. Although the sequencing results are yet to be obtained, identification outcomes of the actinomycetes will be included in the thesis. Results shown above have indicated that actinomycetes have the potentials to inhibit UTI bacteria and further study to identify and characterize the metabolites that are responsible for the inhibition effects could provide more alternatives to manage the UTI disease.