Study of Phenolic Content and Antixoxidant Level In Skins, Seeds and Whole Chilli of Capsicum Frutsescens L (Bird Eye) and Capsicum Annum L

Wong, Kit Yoong (2018) Study of Phenolic Content and Antixoxidant Level In Skins, Seeds and Whole Chilli of Capsicum Frutsescens L (Bird Eye) and Capsicum Annum L. Final Year Project (Bachelor), Tunku Abdul Rahman University College.

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Abstract

Antioxidants can be categoried as substances that can delay or prevent oxidation by stopping formation of free radicals. Antioxidants also interrupt free radical propagation. Antioxidants are compounds usually found in plants. Free radicals are unstable molecules that consist of oxygen that has one or more unpaired electron, leading them to be increasingly reactive in contact with other molecules. Mitochondria which have undergone metabolism produced by-products where were free radical. The objective of this study are to compare the antioxidant properties and phenolic content of Capsicum frutescens L 'Bird's Eye' (Red chili) and Capsicum annuum L (Green chili) using of whole chili, chili seeds and chili skins. The method used for the extractions of the samples was freeze drying method with the use of methanol. Based on the total phenolic content assay, both red and green chili skins consists of higher microgram (μg of gallic acid per gram) of extract (GAE) than their respective chili seeds. The red chili skin (RCSN) showed concentration of 402.222 μg/mL whereas green chili skin (GCSN) shows concentration 350.555 μg/mL. Besides that, the red chili whole (RCW) with the concentration of 725 μg/mL which was higher than green chili whole (GCW) with concentration of 701.667 μg/mL. For total antioxidant capacity (TAC/TAA) assay, RCSN with the concentration 97.454 μg/mL had higher value compared to GCSN with concentration of 96.041 μg/mL. For DPPH radical scavenging, GCSN had the lowest percentage of inhibition which was of 27.46%. Chili skins were higher than seeds in an overall aspect due to their active compound which achieve great antioxidant properties by introducing high phytochemical compounds that agreed with the antioxidant properties. In conclusion, promising potency of chili skin and seeds to be used as nutraceutical and pharmaceutical industry due to their high phytochemical and antioxidant level. KEYWORDS: Capsicum annuum 'Bird's Eye', Capsicum annuum, skins, seeds, Antioxidant properties, Total phenolic content