Effect of Antioxidant Properties on Indian Borage (Coleus aromaticus) Leaves Powder Using Different Drying Methods.

Si, Jia Ying (2019) Effect of Antioxidant Properties on Indian Borage (Coleus aromaticus) Leaves Powder Using Different Drying Methods. Final Year Project (Bachelor), Tunku Abdul Rahman University College.

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Abstract

Indian Borage (Coleus Aromaticus) is an important aromatic herb of the family Lamiaceae which is routinely grown as a traditional medicinal herb in India and South East Asia. The objectives of this study are i) to develop leaves of Indian Borage powder by using hot air oven drying and vacuum oven drying method and ii) to evaluate the effect of drying method and temperature on antioxidant properties physico-chemical properties and antibacterial properties of developed Indian borage leaves powder. Two drying method were tested: i)hot air drying and ii) vacuum drying at two different temperature: 50°C and 55°C. Total phenolic content (TPC), Total flavonoid content (TFC), Vitamin C, 2,2-diphenyl-1-picryhydrazyl (DPPH), FRAP, ABTS, and disc diffusion method were conducted to determine the antioxidant and antimicrobial properties of the developed Indian Borage leaves powder. The results showed that there is a significant difference (p<0.05) which indicated that there is a significant difference between the sample of different drying method. The vacuum oven drying method has higher antioxidant properties as compared to hot air oven drying method. Vacuum oven drying method at 50°C gives a higher percentage of antioxidant activity ferrous ion in ferric reducing antioxidant powder (FRAP) (226.21 ± 8.28). However, vacuum oven drying at 55°C showed the higher percentage of scavenging activity in ABTS (82.59 ± 0.09). The largest zone of inhibition is showed by hot air oven drying method at 55°C (10.2 ± 0.8mm) and Vacuum oven drying at 55°C (10.0 ± 0.5mm) on gram negative bacteria (Escherichia coli). However, there were no significant difference between hot air oven drying and vacuum oven drying. Keywords: Indian Borage leaves powder, Antioxidant properties, Drying method, Hot air oven, Vacuum oven