Determination of Biogenic Amines in Fermented Meat Products Sold in Malaysia by using Reversed-phase High Performance Liquid Chromatography (RP-HPLC)

Liew, Hui Yin (2019) Determination of Biogenic Amines in Fermented Meat Products Sold in Malaysia by using Reversed-phase High Performance Liquid Chromatography (RP-HPLC). Final Year Project (Bachelor), Tunku Abdul Rahman University College.

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Abstract

Nowadays, determination of biogenic amines in fermented food is essential due to its possible toxicity that causes distinctive pharmacological and physiological effect to consumer’s health. Besides, biogenic amines can be used as indicator for quality of freshness or spoilage of foods. The seven most important biogenic amines such as TRP, PEA, HIS, PUT, CAD, TYR and SPD were determined in sixteen selected fermented meat samples that are commonly consumed in Malaysia. Biogenic amines were analyzed after derivatization with dansyl chloride prior to analysis by RP-HPLC with a multi-wavelength UV-Vis Diode Array Detector (DAD). For the ham samples, Ham 1 showed the highest total biogenic amines level which is 100.53 mg/kg. Ham 1 showed an unexpected highest level of TRP (57.45 mg/kg), CAD (16.42 mg/kg) and TYR (5.25 mg/kg). Biogenic amines content showed a relatively low level in hotdog sample which ranged between 48.93 mg/kg to 86.85 mg/kg. For the hotdog (HD) samples, HD 2 has the highest total biogenic amines level which is 86.85 mg/kg and it showed an unexpected highest level of TRP (67.11 mg/kg), HIS (16.21 mg/kg) and CAD (1.80 mg/kg). For the lap chiong (LC) samples, LC 1 has the highest total biogenic amines level which is 169.33 mg/kg and it showed an unexpected highest level of TRP (153.56 mg/kg), SPD (2.66 mg/kg) and CAD (13.11 mg/kg). Biogenic amines content showed a relatively high level in luncheon meat sample which ranged between 152.25 mg/kg to 313.06 mg/kg. For the luncheon meat (LM) samples, LM 3 has the highest total biogenic amines level which is 313.06 mg/kg and it showed an unexpected highest level of PUT (7.12 mg/kg), CAD (5.59 mg/kg), TYR (35.98 mg/kg) and SPD (59.23 mg/kg). TRP was detected in all sixteen fermented meat samples whereas HIS was detected in all fermented meat sample except LC sample. Since the seven biogenic amines content determined in sixteen selected fermented meat sold in Malaysia do not exceed the suggested upper toxicity level, so these meat products are considered safe to be consumed.