Phytochemical Study on the Hexane Extracts of the Berries of Murraya.Koenigii (L.) Spreng and its Antimicrobial Activities

Lim, Shi Min (2019) Phytochemical Study on the Hexane Extracts of the Berries of Murraya.Koenigii (L.) Spreng and its Antimicrobial Activities. Final Year Project (Bachelor), Tunku Abdul Rahman University College.

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Abstract

Murraya koenigii (L.) Spreng is known as curry leaves which is native to India that belonging to family of Rutaceae. The objectives of this research is to isolate antimicrobial compounds from the crude of hexane of the berries of M. koenigii via various chromatographic, such as thin layer chromatography and column chromatography; and spectroscopic methods, such as UV, IR, NMR and polarimeter. The extract and the isolated compounds were evaluated the antimicrobial properties via disc diffusion and microdilution assay. A total of four compounds were isolated from hexane berries extract, and identified as koenimbine, murrayazoline, mahanimbine and cyclomahanimbine. These compounds were displayed good antimicrobial properties through microdilution assay. Based on the microdilution results, koenimbine and mahanimbine are effective in against E. faecium (2.92 mg/mL and 1.25 mg/mL, respectively), S. aureus (same results, 1.25 mg/mL) and E. coli (1.25 mg/mL and 2.50 mg/mL, respectively); whereas murrayazoline and cyclomahanimbine only effective in inhibiting E. faecium (0.08 mg/ml and 0.16 mg/ml, respectively). Therefore, the results showed that M. koenigii (L.) Spreng is a potential source for the development of antimicrobial.