Analysis of Bioactive Compounds of Ethanolic Aerial Extract of Andrographis Paniculata (Nees.) and its Antimicrobial, Antioxidant and Antidiabetic Effects

Hew, Wen Qi (2019) Analysis of Bioactive Compounds of Ethanolic Aerial Extract of Andrographis Paniculata (Nees.) and its Antimicrobial, Antioxidant and Antidiabetic Effects. Final Year Project (Bachelor), Tunku Abdul Rahman University College.

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Abstract

Andrographs paniculata (Nees.) ethanolic aerial extract has been proven to have antimcrobial as well as antoxidant effects and has the potential to be Type 2 diabetes inhibtor. The A.paniculata aerial extract was prepared by polar solvent (absolute ethanol) followed by lyophlizaton. The inhibition zones for Staphylococcus coccus using 60mg/ml and 30mg/ml of A.paniculata aerial extract were found to be 9.0±0.58 mm and 7.0±0.29 mm respectively. For Eschercha col, no inhibition zone was observed. For Bacllus cereus, only 60mg/ml of A.paniculata aeral extract showed an inihibiton zone of 12.5±0.5mm. DPPH assay showed that the percentage of free radical scavenging activity increased proportionally to the concentration of A.paniculata aerial extract. The C50 value was found to be 15.229 mg/ml. The total phenolic content n one gram of A. paniculata aerial extract was found to be 0.2478 mg GAE/ g extract. The boactive compounds n A.paniculata aeral extract were analyzed by Gas Chromatography- Mass Spectrometry (GS-MS). Seven components from A.paniculata aeral extract were identified. The prevailing components in the ethanolic aerial extract of A.paniculata were found to be methyl tetradecanoate (methyl merstate) (8.67%), hexadecanoc acd, methyl ester (36.25%), octadecanoc acid, methyl ester (5.63%), 1,2-benzenedcarboxylc acid (7.52%), 1,1,1,3,5,5,5-heptamethyltrsloxane (6.18%), anthranlic acid, N-methyl-, butyl ester (27.21%) and cyclotrsloxane, hexamethyl- (8.54%). Presence of andrographolde n ethanolic aerial extract of A.panculata was confrmed by HPLC analysis. The C50 value of alpha-glucosdase inhibition was found to be 3.419mg/ml. The presence of various bioactive compound confirms the applicatons of A.paniculata aerial extract for antibacterial and antioxidant properties. The results suggested that A.paniculata (Nees.) ethanolic aerial extract and its boactive compounds have the potential to target disease such as cancer.