The Inhibitory Effect of Polyphenol Oxidase Found in Banana (Musa Spp.) Pulp Extract on Biofilm Formation of Catecholamines Induced Escherichia Coli

Ng, Heng Kang (2019) The Inhibitory Effect of Polyphenol Oxidase Found in Banana (Musa Spp.) Pulp Extract on Biofilm Formation of Catecholamines Induced Escherichia Coli. Final Year Project (Bachelor), Tunku Abdul Rahman University College.

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Abstract

Studies on human health showed that people are more prone to bacterial infection during stressful periods. When the body respond to stress, there will be secretion of stress hormone such as catecholamines to regulate our response and they were believed to have the ability to induce the motility, virulence, biofilm formation of bacteria. However, there were studies found that biofilm formation induced by catecholamines can be inhibited enzymatically. Thus, this project aims to investigate if the polyphenol oxidase (PPO) enzyme found in the banana pulp extract possess the inhibitory effect on biofilm formation of catecholamines induced Escherichia coli. The biofilm formation assay was experimented with the addition of sterile distilled water as control and the addition of banana pulp extract with the ratio of 1:1 (v/v) culture to extract. The culture was incubated for 48 hrs at 37˚C and stained with 1% (w/v) crystal violet stained, dissolved with 100ul dimethyl sulfoxide (DMSO) and measured the absorbance value at 550nm. The absorbance value determines the biofilm formation concentration. The results showed that there is a significant difference (P<0.05) of biofilm formation rate of control compared to the adding of Banana “Tanduk”, Musa x Paradisia fa. Corniculata; Red Banana, Musa acuminata ‘Red Dacca’ and Banana “Rastali”, Musa x paradisiaca. pulp extract. The polyphenol oxidase found in banana pulp extract were hypothesized to have effect on enzymatically degrading the signalling molecule, autoinducer that is essential in quorum sensing during biofilm formation or decrease the adhesion ability during the attachment phase. In conclusion, the polyphenol oxidase found in banana pulp extract showed effect on inhibiting the biofilm formation of catecholamines induced E. coli. Keywords: Biofilm, Catech