1H NMR-based Analysis of Serum Metabolites in Potassium Oxonate-induced Hyperuricemic Rats Treated with Chrysanthemum Morifolium Ramat. Extracts

See, Jing Yi (2019) 1H NMR-based Analysis of Serum Metabolites in Potassium Oxonate-induced Hyperuricemic Rats Treated with Chrysanthemum Morifolium Ramat. Extracts. Final Year Project (Bachelor), Tunku Abdul Rahman University College.

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Abstract

The increment of production or decrement of excretion of uric acid (UA) in the blood results in hyperuricemia (HUA). Potassium oxonate is a selectively competitive uricase inhibitor, which block the effect of hepatic uricase and produces HUA in rodents. In the present study, Chrysanthemum morifolium Ramat. extracts were used as natural xanthine oxidase inhibitor to reduce uric acid level of potassium oxonate induced hyperuricemic rats, with allopurinol as positive control. Rats were divided into vehicle control (normal), positive control, negative control, C. morifolium extract at 25 mg/kg and 50 mg/kg. The urine samples collected on day 6 of the treatment were analysed by 1H nuclear magnetic resonance (NMR) spectroscopy. The visual inspection of the NMR spectra showed that the metabolites profile of the urine in different groups were not significantly different. Therefore, metabolites identification was carried out by comparing the identified metabolite peaks with standard NMR database, Human Metabolome Database and the analysis of the metabolites by multivariate data analysis was used to view in the clustering of treatment groups. There were a total 19 metabolites identified based on HMDB. Based on Principal Component Analysis (PCA), C. morifolium extract at 25mg/kg and positive control were clustered together in the same quadrant of the score scatter plot. Metabolites such as allantoin, uric acid, and creatinine were reduced in 25mg/kg group, similar to that of the positive control. The uric acid reduction property of the 25mg/kg extract indicated that it could be a potential anti-hyperuricemic agent. Therefore, the C. morifolium fraction should be explored further in terms of its cytotoxicity to ensure that it is safe for human consumption.