Studies on the Isolation of Chemical Compounds and Antibacterial Properties of Murraya Koenigii (L.) Spreng Berries Extracts

Lim, Cherng Yiing (2019) Studies on the Isolation of Chemical Compounds and Antibacterial Properties of Murraya Koenigii (L.) Spreng Berries Extracts. Final Year Project (Bachelor), Tunku Abdul Rahman University College.

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Abstract

Murraya koenigii (L.) Spreng is known as the natural medicinal plant as it showed various profound pharmacological activities such as anti-hyperglycemic effect, anti-diabetic, anti-oxidant, antitumor and antimicrobial properties. The objectives of this study is to isolate and elucidate the isolated chemical compounds from berries of M. koeniigi extracts, and also to analyze for its antibacterial properties. Methanolic extract was solvent-solvent partitioned to yield hexane, dichloromethane, ethyl acetate and acetonitrile extracts. These extracts showed the presence of various chemical compounds such as alkaloids and terpenes via phytochemical screening methods. Hexane and dichloromethane crude extracts were found to give positive antibacterial properties on the selected bacterial strains. The crudes were then further analyzed for its chemical properties via chromatographic and spectroscopic techniques. One new compound namely β-caryophyllene oxide (C) is successfully isolated and identified from hexane extract. Besides, two known carbazole alkaloids were isolated from hexane and dichloromethane extracts, which is known as mahanimbine (A) and koenimbine (B). These carbazole alkaloids showed significant activity with MIC value ranging 0.1 to 15.6 μg/mL against E. coli, A. baumanii, and E. faecium when compared to the positive control. The results proved that the Murraya koenigii berries able to provide chemical compounds that gives antibacterial properties which could be useful for the development of new antibiotic drugs.