Analysis of Antioxidant and Antimicrobial Properties of Pometia Pinnata Leaves

Chin, Lee Min (2019) Analysis of Antioxidant and Antimicrobial Properties of Pometia Pinnata Leaves. Final Year Project (Bachelor), Tunku Abdul Rahman University College.

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Abstract

This study evaluated the antioxidant and antimicrobial properties of Pometia pinnata leaves extracted with three different solvents which are water, acetone and methanol. Total phenolic and flavonoid contents of Pometia pinnata leaves were estimated by Folin-Ciocalteau assay and aluminium chloride assay respectively. The antioxidant activity was evaluated by the DPPH free radical scavenging activity. Antimicrobial properties of Pometia pinnata leaves were evaluated using the disc diffusion method to determine the minimum inhibitory concentration against two Gram-positive Staphylococcus aureus and Bacillus cereus, and two Gram-negative Escherichia coli and Proteus mirabilis. Methanol was the most efficient solvent in extracting phytochemical compounds from Pometia pinnata leaves, however, water extract of Pometia pinnata leaves had the highest total phenolic content (73.46 ± 1.20 mg GAE/g extract) and total flavonoid content (20.60 ± 0.89 mg QE/g extract). The water extract also had the highest DPPH free radical scavenging activity value (13.82 ± 0.33 mg AA/g extract). The minimum inhibitory concentration of all the extracts was 2.0 mg/ml and the extracts were able to inhibit only Gram-positive bacteria but not Gram-negative bacteria. The results obtained from this study indicated that the extracts of Pometia pinnata leaves contain potential phytochemical sources to act as antioxidant agents, but have weak antimicrobial properties. Further phytochemical analysis is required for the isolation of phytochemical compounds from the Pometia pinnata leaves to screen for their types and structures.