Phytochemical and Antimicrobial Studies of Zingiber Officinale Roscoe and Zingiber Officinale Var. Bentong

Foong, Shu Ting (2020) Phytochemical and Antimicrobial Studies of Zingiber Officinale Roscoe and Zingiber Officinale Var. Bentong. Final Year Project (Bachelor), Tunku Abdul Rahman University College.

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Abstract

Ginger (Zingiber officinale) is a perennial herb that belongs to the family Zingiberaceae. It has notable pharmacological properties to humans such as antioxidant, antimicrobial, anti-carcinogenic and anti-inflammatory activities. The project aimed to study the phytochemical screening of ginger, to isolate and characterize the pure compounds from various types of ginger. In this project, the Z. officinale var. Bentong, Z. officinale Roscoe (Old) and Z. officinale Roscoe (Young) were dried and extracted using a Soxhlet extraction method with acetone as the solvent. The dried crude extracts were then analysed for the antimicrobial activities and total phenolic content assay. The chromatographic and spectroscopic techniques were carried out for purification, isolation and characterization of the isolated components from Z. officinale var. Bentong. The results showed that the Z. officinale var. Bentong exhibited the lowest moisture content (88.40%) with the highest extraction yield (10.43%) among the selected ginger samples. For the UHPLC profiling, Z. officinale var. Bentong was also found to contain the highest amount of components such as [6]-gingerol, [10]-gingerol, [6]-shogaol and [8]-shogaol when compared to others. Also, the Z. officinale var. Bentong has effectively inhibited against A. baumannii with MIC of 625 g/mL. Lastly, two pure compounds, [6]-gingerol and benzene-1,2,4-triol were successfully isolated from Z. officinale var. Bentong. These compounds were elucidated using NMR analysis by comparison with the literature data. Benzene-1,2,4-triol was found to be the first time isolated from the plant. This plant has shown its potential to provide natural bioactive compounds for future investigation.