Effect of Bioactive Polysaccharide Extracted from Flammulina Velutipes (Enokitake) and Lentinula Edodes (Shiitake) Mushroom on the Physicochemical and Functional Properties of Gelatin Edible Film

Koh, Kar Wei (2018) Effect of Bioactive Polysaccharide Extracted from Flammulina Velutipes (Enokitake) and Lentinula Edodes (Shiitake) Mushroom on the Physicochemical and Functional Properties of Gelatin Edible Film. Final Year Project (Bachelor), Tunku Abdul Rahman University College.

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Abstract

This study was carried out to investigate the effect of the incorporation of bioactive polysaccharide extracted from Flammulina Velutipes (Enokitake) and Lentinula Edodes (Shiitake) mushroom on the physicochemical and functional properties of gelatin film. First of all, the optimum polysaccharide extraction condition for both mushrooms were determined. Based on the results obtained, Shiitake extract produced from extraction at substrate to solvent ratio of 1:25 for 2 hours, and Enokitake extract produced from extraction at substrate to solvent ratio of 1:25 for 1 hour, had the highest DPPH radical-scavenging activity. Under the optimum condition, the produced Shiitake and Enokitake extract contained 0.062 ± 0.010g and 0.144±0.090g of total carbohydrate respectively and possessed antioxidant capacity at 91.03 ± 11.30 ppm and 42.05±6.23ppm of ascorbic acid equivalent respectively. The polysaccharide containing extract was then incorporated into the film forming mixture containing gelatin and glycerol. The films were produced by substituted distilled water with 50% (v/v) and 100% (v/v) extract respectively. Gelatin film without extract was used as the experiment control. Results of texture profile analysis indicated that by substituting 50% Shiitake extract and 100% Enokitake extract, the produced gelatin film possessed the highest tensile strength at 0.133 ± 0.397 MPa, and 0.108 ± 0.036 MPa respectively. The highest percentage of elongation at break was observed in both film containing 100% extract (247.5 ± 61.9 % for Shiitake and 221.01 ± 4.19 % for Enokitake). Gelatin film produced by substituting 100% distilled water with Shiitake extract was found possess the highest amylase inhibition activity.