Bioactivities of the Edible Bird Nest Hydrolysates Produced by Proteinases

Lok, Yeu Yang (2018) Bioactivities of the Edible Bird Nest Hydrolysates Produced by Proteinases. Final Year Project (Bachelor), Tunku Abdul Rahman University College.

Text Lok Yeu Yang_Full Text.pdf Restricted to Registered users only Download (746kB)

Abstract

Edible birds nest (EBN) is a valuable functional food and is believed to have a therapeutic function in herbal medicine. Most EBN found in Malaysia is from the swiflet species, Aerodromus fuciphagus. The most abundant amino acids in the nest are serine, threonine, aspartic acid, glutamic acid, proline, and valine. The objectives of this study were to determine the hydrolysis time in producing the highest bioactivity and the antioxidant activities of EBN hydrolysates. Edible bird nest protein hydrolysates (EBN) were prepared via enzymatic hydrolysis to investigate its antioxidant activity. Two types of enzyme (alcalase and papain) were used in this study and EBN had been hydrolysed with different hydrolysis time (30, 60, 120 and 180 min). Antioxidant activities in EBN protein hydrolysate were measured using DPPH and Ferric Reducing Antioxidant Power Assay. From this study, for antioxidant assay, EBN hydrolysed with papain showed higher reducing power ability compared to alcalase while alcalase shown higher scavenging activity compared to papain. The highest antioxidant activity for papain was at 120 min hydrolysis time with FRAP assay (Ferrous Equivalent=70.429±0.756a mg/L) and DPPH assay (Ascorbic acid Equivalent= 53.8.98±0.927a mg/L). While for alcalase 2 (liquid form), the highest antioxidant activity was at 120min hydrolysis time with with FRAP assay (Ferrous Equivalent= 68.429±0.247amg/L) and 60min hydrolysis time with DPPH assay (Ascorbic acid Equivalent= 63.310±0.760amg/L). Besides that, the peptide content of EBN hydrolysates with different time interval incubated by papain and alcalase were also evaluated.