Comparative Evaluation and Determination of Anti-Oxidant and Anti-Inflammatory Property of Calathea Lutea in Different Solvent and Drying Method

N. Chandran, Saranya (2020) Comparative Evaluation and Determination of Anti-Oxidant and Anti-Inflammatory Property of Calathea Lutea in Different Solvent and Drying Method. Final Year Project (Bachelor), Tunku Abdul Rahman University College.

Text Saranya N Chandran_Full Text.pdf Restricted to Registered users only Download (2MB)

Abstract

Calathea lutea belongs to the order of Zingiberales (ginger and turmeric) native to the tropical Americans which is also commonly called as “Cuban cigar”. The objectives of this research is to determine the anti-oxidant property and anti-inflammation property in aqueous and ethanol extract of Calathea lutea. The research was initiated by drying the fresh rhizome of Calathea lutea using freeze-dried and oven-dried method and suspending it in aqueous and 95% ethanol solvent for maceration. The phytochemical screening of the solvent extractions were carried out, followed by the determination of phenolic content (TPC), flavonoid content (TFC), radical scavenging activity (DPPH) and LOX-inhibition test. All the test samples were done in triplicate and all the data are expressed in mean± standard deviation using One-way ANOVA test (p<0.05). The results of percentage of dried yield had shown that freeze-dried sample had given higher yield (27.61%) compared to oven dry. In phytochemical screening, the presence of polyphenols , steroids, terpenoids ,saponin and triterpenes were examined and the results proves that almost all the secondary metabolites except hydrolysable tannin was present. The aqueous extract exhibiting better phytochemical results than ethanol extract due to the high polar nature of aqueous solvent compared to ethanol. The ethanolic extract shown highest content of TPC and TFC (976.71mg GAE/g and 1132.16 mg QE/g). The radical scavenging activity (IC50) of all the extraction was higher than ascorbic acid (37.1ppm) and oven dried-aqueous extraction had the highest IC50 value (46.9ppm) and FRS value of 87.89mgAA/g. LOX inhibition test showed the oven-dried ethanol extraction has higher IC50 value (48.9ppm) compared to positive control, ibuprofen (45.7ppm). On the whole, Calathea lutea exhibits anti-oxidant and anti-inflammatory property and this plant has a scope for further researches in future to determine other therapeutic effect of this species.