Phytochemistry and Antimicrobial Properties of Portulaca oleracea sp.

Lye, Jia Yan (2021) Phytochemistry and Antimicrobial Properties of Portulaca oleracea sp. Final Year Project (Bachelor), Tunku Abdul Rahman University College.

Text Lye Jia Yan_Full Text.pdf Restricted to Registered users only Download (1MB)

Abstract

A substantial increase in demands for nutraceutical products as antimicrobial and therapeutic alternatives has resulted in a long and growing list of research on plants with beneficial phytochemical properties. Portulaca oleracea sp, generally recognized as an annual, prostrate, succulent weed with worldwide distribution that has long been used as a nutrition diet and therapeutic plant. Purslane always grows rapidly and has the acceptance of, light intensity, temperature, and moisture soil type. Portulaca oleracea provides a large spectrum of pharmacologic properties similar to neuro-protective, antibacterial, anti-fungal, antidiuretic, antioxidant activity, anti-inflammatory acitivities, anti-ulcerogenic, and metastatic tumor activities that are related to its abundance of phytochemicals. This project aims to arrange for a transitory overview of the antibacterial activity with the phytochemistry of Portulaca oleracea. Based on analysis using GC-MS, HPLC, and LC-MS/MS, various complexes had remote from Portulaca o plants, which have different types of flavonoids, different type of alkaloids, several phenolic acids, betanin, terpenoids, and etc. Wild purslane has a higher abundance of several phytochemicals compared to cultivated species. As mentioned, microwave-assisted extraction has the peak extraction efficiency among to maceration, ultrasound extraction, and Soxhlet extraction in extracting flavonoids from P. oleracea. Chamber-grown purslane contained an exceptionally high amount of omega-3 fatty acids. Significantly higher levels of phenolic compounds and antioxidant capabilities were obtained with ethanolic extracts, compared with H2O extracts in both leaves and stems. Chloroform and ethanolic extraction from the aerial part of Portulaca oleracea L showed antimicrobial effects towards Staphylococcus aureus, Bacillus cereus, Klebsiella pneumoniae, Neurospora curcus, and Aspergillus fumigates while Pseudomonas aeruginosa was resistant. Chloroform extract was ineffective against Escherichia coli. In the preliminary study, cold maceration with solvent of 90% methanol in room temperature of 25°C and soxhlet extraction were done with 90% methanol in 7 hours continuously. Disk diffusion assays did not show zones of inhibition in contradiction of Staphylococcus aureus and Escherichia coli for the extracts of plants at a concentration of 50mg/ml with the solvent of methanol. Further tests will be needed to investigate the antimicrobial activity. This much-overlooked phytochemical-rich plant should deserve major consideration as an edible vegetable.