Phytochemical Profiling and Antioxidant Properties of Tradescantia Spathacea SW. Roots

Chia, Xin Yue (2022) Phytochemical Profiling and Antioxidant Properties of Tradescantia Spathacea SW. Roots. Final Year Project (Bachelor), Tunku Abdul Rahman University College.

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Abstract

Tradescantia spathacea Sw. is a natural medicinal plant due to its various physiological properties such as antioxidant, anti-inflammatory, antimicrobial, anticancer, and antiviral activities. In this research project, the roots of T. spathacea were chosen to be studied as there is a lack of information being reported on its phytochemicals and antioxidant properties. The objectives of this study are to identify the possible phytochemical constituents present in the roots extracts and to evaluate the antioxidant activity of the crude extracts. Phytochemical screening conducted showed the presence of alkaloids, flavonoids, terpenes, quinones, and glycosides in the crude extracts, and the fourier transform infrared (FT-IR) spectrometric screening also revealed the presence of phenols. Two possible volatile compounds were reported for hexane crude which was known as 2(1H)-Pyrimidinone, 5-chloro-4,6-diphenyland 5-(p-Aminophenyl)-4-(p-tolyl)-2-thiazolamine by gas chromatography-mass spectroscopy (GC-MS) volatile component profiling. It was observed that hydroethanolic crude contained the highest total phenolic content (383.1 ± 7.2 mg GAE/g dry extract). The ethyl acetate crude was reported to have higher total flavonoid content (277.3 ± 2.4 mg CE/g dry extract) and higher antioxidant activity (6.13 ± 0.90 mmol Fe2+/g dry extract) in ferric reducing antioxidant power (FRAP) assay, comparing to the hexane crude. However, hexane crude also showed higher antioxidant properties in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay with a lower half maximal inhibitory concentration determined (IC50 = 70 μg/mL). Therefore, the results obtained proved that the compounds from T. spathacea roots have the potential to be developed as an antioxidant medication