Comparative Anticancer and Cytotoxicity Studies of Ternary Metal Complexes [M(phen)(edda)] [M(II) = Cu, Zn] towards Melanoma Cell Line (SK-MEL-5) with Different Expression of EZH1

Tan, Chee Teong (2022) Comparative Anticancer and Cytotoxicity Studies of Ternary Metal Complexes [M(phen)(edda)] [M(II) = Cu, Zn] towards Melanoma Cell Line (SK-MEL-5) with Different Expression of EZH1. Final Year Project (Bachelor), Tunku Abdul Rahman University College.

Text TAN CHEE TEONG_Full Text.pdf Restricted to Registered users only Download (1MB)

Abstract

Melanoma is a type of skin cancer characterized by dark pigmentation of harmful neoplasm on skin melanocytes and it is a fast-growing skin cancer. Over the years, researchers were exploring the usage of metal complexes in cancer treatment and promising results had been developed. Copper(II) complex and zinc(II) complex have been great interests of researchers since they exhibit significant cytotoxic effect towards cancer cells. EZH1 which forms the catalytic site of PRC2 complex was associated with the silencing of tumour suppressor genes in cancer cells increasing the proliferation of malignant tumours. The overexpression of EZH1 is believed to increase the resistance of cancer cells towards drug and metal complex. In this research, the anticancer potency and cytotoxicity of cisplatin, Cu(phen)(edda) and Zn(phen)(edda) were evaluated and compared against SK-MEL-5/vector and SK-MEL-5/EZH1. Cisplatin was set as the positive control. Cell viability for SK-MEL-5 treated with a series of concentrations ranges from 0.1 M to 100 M of the three metal complexes were determined via MTT assay. Among SK-MEL-5/vector, the GI50, TGI and LC50 of Cu(phen)(edda) were significantly lower than cisplatin and the LC50 of Cu(phen)(edda) was significantly lower than Zn(phen)(edda). The GI50 and TGI of Zn(phen)(edda) were significantly lower than cisplatin but its LC50 was significantly higher than cisplatin. Among SK-MEL-5/EZH1, the GI50, TGI and LC50 of Cu(phen)(edda) were significantly lower than two other complexes. The TGI and LC50 of cisplatin were significantly lower than Zn(phen)(edda). Thus, Cu(phen)(edda) exhibits highest anticancer potency and cytotoxicity among the complexes. In addition, SK-MEL-5/vector showed significantly lower GI50, TGI and LC50 towards Zn(phen)(edda) as compared to SK-MEL-5/EZH1 indicating that the overexpression of EZH1 had increased the resistance of SK-MEL-5/EZH1 towards Zn(phen)(edda). No significant difference was observed in SK-MEL-5/EZH1 towards Cu(phen)(edda) as compared to SK-MEL-5/vector