Effect of Ultrasound Treatment on the Performance of Enzymatic Hydrolysis and Bioactivity of Edible Bird’s Nest

Koh, Xian Jian (2022) Effect of Ultrasound Treatment on the Performance of Enzymatic Hydrolysis and Bioactivity of Edible Bird’s Nest. Final Year Project (Bachelor), Tunku Abdul Rahman University College.

Text Koh Xian Jian_Full Text.pdf Restricted to Registered users only Download (3MB)

Abstract

Enzyme technology has led to the innovation of edible bird’s nest hydrolysate (EBNH). Ultrasound (US) is one of the promising advanced technologies to enhance the performance of enzymatic reaction. This project aims to investigate the effect of US amplitude and time on the chemical, physicochemical and bioactive properties of EBNH. In this study, 4 % w/v of grade E raw EBN was subjected to US treatment at different amplitudes (30,60 and 90 %) and times (15, 30 and 45 min) prior to papain hydrolysis (pH 7, 60 °C, 90 min and 150 rpm). Then, the optimum US amplitude and time was selected to proceed with phase 2 optimization to study the effects of different enzymatic hydrolysis times (60,90 and 120 min) on the chemical physicochemical and bioactive properties of EBNH. Results suggest that US amplitude had a more significant effect than US treatment time in lowering the viscosity of EBNH. After US treatment, the viscosity was reduced from 29.01± 1.61 mPa·s (EBNH without US treatment) to 6.58 ± 0.10 mPa·s (EBNH sonicated for 30 min at amplitude of 90 %). However, US treatment did not significantly affect the hydrolysis degree, total soluble protein and DPPH scavenging activity of EBNH. But, it had positive effects on lipoxygenase inhibitory activity and α-amylase inhibitory activity of EBNH. It was concluded that 30 min of US treatment at amplitude of 90 % with combination of 90 min papain hydrolysis were the optimum treatment conditions to produce EBNH in this study. EBNH produced under this condition has a viscosity of 6.58 ± 0.10 mPa·s, 1.58 ± 0.16 % of hydrolysis degree, 18.63 ± 2.38 mg BSA/ml of total soluble protein, 11.27 ± 1.15 % of DPPH scavenging activity, 61.66 ± 2.89 % of lipoxygenase inhibitory activity and 77.40 ± 5.41 % of α- amylase inhibitory activity. This study recommends US technology as the promising pretreatment technique in EBNH production