Effect of Extraction Methods on the Chemical and Bioactive Properties of Durian Rind Polusaccharides

Teh, Won Way (2023) Effect of Extraction Methods on the Chemical and Bioactive Properties of Durian Rind Polusaccharides. Final Year Project (Bachelor), Tunku Abdul Rahman University of Management and Technology.

Text Teh Won Way_Full Text.pdf Restricted to Registered users only Download (745kB)

Abstract

This research was carried out to investigate the effect of extraction methods on the yield and antioxidant and anti-inflammatory activities of durian rind polysaccharides obtained from the durian of Musang King species. Initially, durian rind polysaccharides (DRP) were extracted using hot water extraction (HWE) method and enzyme-assisted ultrasound extraction (EAUE) method respectively. The crude durian rind polysaccharides (CDRP) were characterized by the dry weight of CDRP, polysaccharide recovery, total soluble polysaccharide yield, FT-IR, antioxidant and anti-inflammatory activities. The total CDRP extracted from HWE (H) and EAUE (E) methods were 195.01g and 128.75g respectively. The greater production of CDRP from HWE method may be the reason that leads to the greater polysaccharide recovery (31.73%) and total soluble polysaccharide yield (2.09%). Antioxidant activities were evaluated using DPPH radical scavenging assay, ABTS cation radical scavenging assay, and FRAP assay while albumin denaturation inhibition activity was conducted to determine anti-inflammatory activity. The molecular and structural characteristics of H and E were determined using FT-IR. FT-IR spectrum showed that pectin was present in H and E samples. Overall, the results showed that H exhibited higher DPPH (52.43±0.86%), ABTS (80.78±1.26%), FRAP assay (452.0 ± 15.31 FRAP mg/100g) and albumin denaturation inhibitory activity (44.53±1.46%) than E. In conclusion, hot water extracted durian rind polysaccharide was proven to be a great bioactive ingredient for functional food application. Keywords: Durian rind polysaccharides, extraction, enzyme-assisted ultrasound extraction, antioxidant, anti-inflammatory