Antioxidative, Anti Inflammatory and Cytotoxic Effects of Standardized Papaya Leaves Fraction

Ng, Suet Ying (2024) Antioxidative, Anti Inflammatory and Cytotoxic Effects of Standardized Papaya Leaves Fraction. Final Year Project (Bachelor), Tunku Abdul Rahman University of Management and Technology.

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Abstract

The Carica papaya Linn. leaves fractions were proven to have therapeutic application in the management of human diseases. The main aim of this study are 1) To investigate the antioxidant activity of papaya leaves fractions by DPPH and ABTS radical scavenging assay, 2) To determine the anti-inflammatory effect of papaya leaves fractions by anti-lipoxygenase assay and 3) To standardize the linolenic acid of papaya leaves fractions. Ethyl acetate (EA) fraction showed stronger ABTS and DPPH radical scavenging activities than Petroleum Ether (PE) fraction. The EA fraction also exhibited higher inhibition of lipoxygenase enzyme than PE fraction by around 1 fold at the concentration of 200 μg/ml. Both fractions showed non toxicity towards HepG2 liver cells at concentration 5 to 40 μg/ml. Interestingly, ROS assay conducted indicated that PE fraction exerted better ROS reducing potential in oxidative stressed liver cells than EA fraction. This could be contributed by the phytochemical constituents that are present in the PE fraction. Molecular docking and dynamic experiments conducted by our collaborators indicated that linolenic acid has a positive effect on Nrf2 activation which could improve liver health. GC-MS analysis identified that linolenic acid was the second most abundant compound in PE fraction. Linolenic acid in PE fraction was quantified to be 62.6481 ± 0.9170 μg/ml utilizing HPLC and could be the phytocompound responsible for the biological activity. In view of that, more investigation should be conducted on linolenic acid to validate the findings.