Induction of Chitinase Activity and Comparison of Growth of Three Endophytic Bacteria Strains: SL14 (1), SL14 (2), and YC

Too, Wei Kang (2024) Induction of Chitinase Activity and Comparison of Growth of Three Endophytic Bacteria Strains: SL14 (1), SL14 (2), and YC. Final Year Project (Bachelor), Tunku Abdul Rahman University of Management and Technology.

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Abstract

Inoculation of bacterial strains into the soil environment has proven itself to be an effective and reliable technique for enhancing plant growth and yield by means of increasing nutrient uptake by plants and defending them from plant diseases. This project aims to understand and compare the ability of three endophytic bacteria, namely SL14 (1), SL14 (2) and YC, which were previously shown to inhibit the growth of Ganoderma boninense, to produce chitinase enzyme after induction in a chitin-containing medium and to grow in soil. Chitinase activity was induced in all bacteria strains via inoculation in a chitin-supplemented minimal salt medium (MSM) for 30 days. The results show chitinase activity induced in SL14 (1), which showed no chitinase activity before induction, was similar to that of SL14 (2) and YC before induction. Chitinase activity in SL14 (2) and YC both increased after the induction to 0.52 fold and 0.41-fold respectively. Based on the growth curve, SL14 (1) and YC exhibited fast growth in LB broth medium, whereas SL14 (2) exhibited slow growth. Growth of bacteria was measured as CFU (colony forming unit) after inoculation in sterile soil for 5 days at room temperature. The CFU value of the post-inoculation SL14 (1) strain was calculated to be 1.56 x 105 CFU/mL, whereas the CFU values for the SL14 (2) and YC were 2.07 x 106 CFU/mL and 1.06 x 106 CFU/mL, respectively, indicating that SL14 (2) exhibited the greatest ability to grow and proliferate in the soil, with YC cultures being the second fastest-growing, and SL14 (1) being the slowest.