Effects of Enzyme Pretreatment on the Chemical and Bioactive Properties of Durian Rind Polysaccharides

Ling, Zi Yi (2024) Effects of Enzyme Pretreatment on the Chemical and Bioactive Properties of Durian Rind Polysaccharides. Final Year Project (Bachelor), Tunku Abdul Rahman University of Management and Technology.

Text Ling Zi Yi_Full Text.pdf Restricted to Registered users only Download (1MB)

Abstract

The present study investigated the effect of enzyme pretreatment on the chemical and bioactive properties of durian rind polysaccharides obtained from Sultan Durian (D24) species originating in Malaysia. Durian rind polysaccharides (DRP) were extracted using an enzyme-assisted hot water extraction technique. Polysaccharides extracted through hot water extraction (WP) served as the experiment control. Pectinase-extracted polysaccharides (PP) were produced via pectinase-assisted hot water extraction, whereas cellulase-extracted polysaccharide (CP) were produced through cellulase-assisted hot water extraction. The chemical (moisture content, yield, purity, soluble polysaccharide, sugar content, FTIR) and bioactive (ABTS, FRAP, albumin denaturation inhibition activity, anti-tyrosinase activity, and prebiotic property) characteristics of WP, PP, and CP were evaluated. Among the extraction methods, cellulase-assisted hot water extraction produced the highest polysaccharide (CP) yield at approximately 74.8 %. However, PP was found to contain the greatest total soluble polysaccharide content (38.8 ± 8.2 %). Compared to PP, CP exhibited higher ABTS, FRAP, albumin denaturation inhibition activity and anti-tyrosinase activity. The FT-IR spectrum proved that WP, PP, and CP resembled chemical structures, all being pectic polysaccharide. Additionally, the study also demonstrated that WP, PP and CP enhanced Lactiplantibacillus plantarum TAR 4 growth, confirming their prebiotic properties, with no significant difference among WP, PP and CP. This pioneer study provides new insight into the advantage of cellulase pretreatment in enhancing bioactive properties of DRP. The study proved that CP potentially emerged as a novel bioactive polysaccharide with antioxidant, anti-inflammatory and skin whitening activities.