Evaluation of Therapeutic Activities of Postbiotic Derived from Lactiplantibacillus Plantarum TAR4 with a Focus on Antioxidant, Antidiabetic, and Anti-Biofilm Properties

Tang, Meiki (2025) Evaluation of Therapeutic Activities of Postbiotic Derived from Lactiplantibacillus Plantarum TAR4 with a Focus on Antioxidant, Antidiabetic, and Anti-Biofilm Properties. Final Year Project (Bachelor), Tunku Abdul Rahman University of Management and Technology.

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Abstract

Inflammatory bowel diseases (IBD) and colorectal cancer (CRC) are chronic gastrointestinal disorders related to gut dysbiosis. Postbiotics, the inanimate forms and metabolites produced by probiotics during their growth, have gained considerable interest as alternatives to probiotics for their safer and more stable properties. This research aimed to evaluate the antioxidant, antidiabetic and anti-biofilm properties of postbiotics cell-free supernatant (CFS) and cell lysate derived from L. plantarum TAR4. The antioxidant, antidiabetic and anti-biofilm properties of these postbiotics were evaluated via DPPH and ABTS radical scavenging assays, α-amylase assay and biofilm inhibition against E. coli ATCC 25922 and L. monocytogenes 102975 using crystal violet assay. The study revealed that cell lysate of L. acidophilus ATCC 4356 displayed a significantly lower IC50 in DPPH assay as compared to 1 mg/mL ascorbic acid (p<0.05). In ABTS assay, CFS of L. plantarum TAR4 and L. acidophilus ATCC 4356 displayed 99.88% and 99.35% ABTS radical scavenging activity in comparison to 1 mg/mL Trolox (56.79%) with significant difference (p<0.05). Cell lysates of both L. plantarum TAR4 and L. acidophilus ATCC 4356 showed a significantly lower IC50 in comparison to 1 mg/mL Trolox (p<0.05). Notably, CFS of L. plantarum TAR4 exhibited a significantly (p<0.05) higher α-amylase inhibition (91.30%) as compared to L. acidophilus ATCC 4356 (74.85%) and 1 mg/mL acarbose (75.24%). The CFS of L. plantarum TAR4 and L. acidophilus ATCC 4356 showed 17.41% and 18.02% biofilm inhibition against L. monocytogenes 102975 while 1 mg/mL ampicillin showed no effect (p<0.05). CFS of L. plantarum TAR4 displays the most promising antioxidative, hypoglycemic and anti-biofilm properties, making it a potential pharmaceutical candidate for preventing oxidative damage, diabetes and modulating gut microbiota. Keywords: Postbiotics; Lactiplantibacillus plantarum TAR4; Cell-free supernatant; Cell lysate; Bioactivity