The Bioactive Properties of Lactiplantibacillus Plantarum TAR4 Derived Postbiotics and Durian Rind Polysaccharide Extract as Potential Functional Ingredients for Cosmetic Applications

Ruben, Wisanggeni Johanes (2026) The Bioactive Properties of Lactiplantibacillus Plantarum TAR4 Derived Postbiotics and Durian Rind Polysaccharide Extract as Potential Functional Ingredients for Cosmetic Applications. Final Year Project (Bachelor), Tunku Abdul Rahman University of Management and Technology.

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Abstract

Driven by consumer demand for safer products and environmental responsibility, the global cosmetic industry is shifting towards more natural and sustainable ingredients. This project aims to evaluate the bioactive potential of durian rind polysaccharide extract and Lactiplantibacillus plantarum TAR4 derived postbiotics, and to assess their applicability as functional cosmetic ingredients. L. plantarum TAR4 was first grown onto MRS agar and inoculated into MRS broth. The inoculated broth was left to grow for 48 hours and centrifuged at 4000 rpm for 10 minutes at 4 °C. The supernatant was filtered with 0.45 μm sterile PES syringe filter to obtain a cell-free supernatant (CFS). Durian rind polysaccharide extract (DP) was mixed with absolute ethanol at a ratio of 1:3, left to stand at room temperature overnight, and centrifuged. The extracts were then freeze dried and used for assay along with the CFS, and respective positive controls. Bioactivities were evaluated through anti-oxidant, anti-inflammatory, and anti-tyrosinase assays. Results showed significant differences between each sample (p < 0.05). CFS showed IC₅₀ values of 19.33 ± 0.81% v/v (DPPH), 7.31 ± 0.37% v/v (ABTS), 52.13 ± 0.22% v/v (anti-tyrosinase), and 30.44 ± 0.11% v/v (anti-inflammatory). DP did not exhibit measurable activity in the DPPH, anti-tyrosinase, or anti-inflammatory assays. However, DP demonstrated ABTS scavenging at IC₅₀ = 7.31 ± 0.37 mg/mL). While DP showed limited activity against several assays, L. Plantarum postbiotic showed broader functional effects, which suggest the potential application of bacterial postbiotic as sustainable cosmetic bioactive, while emphasizing the selective application of durian rind polysaccharide. The project aligned with the United Nation’s Sustainable Development Goals (SDG) 9 on Industry, Innovation, and Infrastructure and SDG 12 on Responsible Consumption and Production.