Biosafety Assessment of Listeria Monocytogenes in Sliced Fruit by Using MPN-Plating and MPN-PCR Methods

 




 

Teng, Sui Ni (2016) Biosafety Assessment of Listeria Monocytogenes in Sliced Fruit by Using MPN-Plating and MPN-PCR Methods. Final Year Project (Bachelor), Tunku Abdul Rahman University College.

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Abstract

People are now consuming more fruits in Malaysia as it contains a lot of essential nutrients for human health. The incidence of Listeria monocytogenes outbreaks associated with fruits especially the sliced fruits has drawn concern from public. The aims of this study were to compare the MPN-PCR and MPN-plating methods in term of detection of L. monocytogenes and the prevalence of L. monocytogenes in different sliced fruits samples. A total of 24 fruits samples were examined for the prevalence and microbial load of L. monocytogenes by using the MPN-PCR and MPN Plating methods. The samples were collected from two different hawker stalls in Setapak, Kuala Lumpur including mango, pineapple, honeydew, guava, papaya, and watermelon. For fruits samples, L. monocytogenes was detected at a prevalence of 74.3% and 64.6%, using MPN-PCR and MPN-plating methods, receptively. The estimated count of L. monocytogenes were ranged from 4.5 to 1000 MPN/g and <3 to 430 MPN/g by using MPN-PCR and MPN-Plating, receptively. The recovery rate of L. monocytogenes in MPN-Plating was estimated to be 44.4 %. The result of this study showed the MPNPCR method is more accurate and reliable as compared with MPN-Plating method. It was concluded that MPN-PCR method could be considered as an alternative to conventional MPN methods. Besides that, this study also indicates that the sliced fruit may pose a potential risk of listeria infection. Key word: Listeria monocytogenes, prevalence, Most Probable Number (MPN), Polymerase Chain Reaction (PCR), Plating, sliced fruits.

Item Type: Final Year Project
Subjects: Technology > Food Technology
Faculties: Faculty of Applied Sciences and Computing > Bachelor of Science (Honours) in Food Science
Depositing User: Library Staff
Date Deposited: 07 Aug 2019 03:22
Last Modified: 19 Oct 2020 08:53
URI: https://eprints.tarc.edu.my/id/eprint/4128