Screening of Enzymes Activity and Heavy Metals Removal Potential from Fungal Isolated

Lim, Shi Yun (2016) Screening of Enzymes Activity and Heavy Metals Removal Potential from Fungal Isolated. Final Year Project (Bachelor), Tunku Abdul Rahman University College.

Text Lim Shi Yun_FULL TEXT.pdf Restricted to Registered users only Download (1MB)

Abstract

This study is to isolate some fungi from rotten vegetable to produce useful enzymes for commercial purpose and study the biosorption capacity of the fungi. Penicillium sp., Penicillium roqueforti, Aspergillus sp. 1, Aspergillus sp. 2, Aspergillus sp. 3 and Aspergillus sp. 4 were isolated from the rotten vegetables. In this study, the extracellular enzymes screening tests were performed for all six strains of fungi and lead (II) ions biosorption by biomass from three black molds and Aspergillus sp. 1 was performed. All the fungi isolated were able to produce the extracellular enzymes, where the Penicillium roqueforti had produced the highest -galactosidase and cellulase. The fungus with the highest xylanase acitivity was Aspergillus sp. 3 isolated from onion. The fungus with the highest lead removal efficiency was used for adsorption isotherm and kinetic studies. The lead removal efficiency of four different strains of fungi were carried out at the experimental conditions of initial lead (II) concentration 50mg/L, biomass dose 2g/L, contact time 24 hours, agitating speed 150rpm and room temperature. The remaining lead (II) ions in the samples were measured by using Flame Atomic Absorption Spectrophotometer. At pH 4.0 ± 0.1, the Aspergillus sp. 1 had the highest lead (II) removal efficiency among all four strains; the removal amount was 42.585 ± 0.000 mg/g. The biosorption equilibrium for Aspergillus sp. 1 cost about two hours at experimental conditions of pH 4.0 ± 0.1, initial lead (II) concentrations ranged from 20mg/L to 100mg/L, biomass dose 2g/L, contact time 12 hours, agitating speed 150rpm and room temperature. The Aspergillus sp. 1 possesses high biosorption capacity for the lead (II) ions and Freundlich isotherm model and Pseudo-second order kinetic model can used to describe the reaction better than the other models were used in this study.