In Vitro Xanthine Oxidase Inhibition by Chrysanthemum Morifolium and Its Mode of Inhibiton

Ng, Teng Lit (2017) In Vitro Xanthine Oxidase Inhibition by Chrysanthemum Morifolium and Its Mode of Inhibiton. Final Year Project (Bachelor), Tunku Abdul Rahman University College.

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Abstract

Gout disease has been well-known for its effects to human health. Natural products are one of the excellent sources of lead compounds in the search of new medication for clinical disorders. In the present study, various fractions of the hydromethanolic extract of the flower of Chrysanthemum morifolium was evaluated for its Xanthine Oxidase (XO) inhibitory potential. Its aqueous fractions extracts, which are crude, ethyl acetate (EA), petroleum ether (PE) and residual fractions (RF), were tested in vitro for their inhibition potencies expressed as % inhibition of XO activity. Enzyme kinetics was carried out using Lineweaver-Burk plots using xanthine as the substrate. Among the fractions tested, the EA fraction exhibited highest potency of 49.55±0.92%; IC50 = 10.86±0.86μg/mL, followed by the RF fraction, 28.55±5.58%; IC50 =101.77±13.44μg/mL, PE fraction, 22.79±8.22%; IC50 = 58.07±10.79μg/mL and crude fractions, 16.58±3.35%; IC50 value = 150.11±12.30μg/mL. The IC50 value for allopurinol (positive control) was 9.85±0.15μg/mL. Enzyme inhibition mechanism indicated that the mode of inhibition for RF fraction was of competitive inhibition whereas the crude, EA, and PE were non-competitive type of inhibitions. The results indicated that C. morifolium can serve as a potential source of natural XO inhibitors that can be elaborated as herbal remedies for gout, arthritis and other XO-related disorders