The Effect of Organic Fertilizer Against Disease Susceptibility of Sweet Basil (Ocimum Basilicum) to Pseudomonas Aeruginosa

Liew, Chea Yang (2018) The Effect of Organic Fertilizer Against Disease Susceptibility of Sweet Basil (Ocimum Basilicum) to Pseudomonas Aeruginosa. Final Year Project (Bachelor), Tunku Abdul Rahman University College.

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Abstract

Pseudomonas aeruginosa is a ubiquitous Gram-negative, rod shaped bacteria that can survive on habitat surfaces by forming biofilm and thus is impervious to a lot of antibacterial reagents. P.aeruginosa can initiate infection by invading the host defense system. Since the application of organic fertilizer is known to improve disease resistance of plant, this project was carried to study the effects of application of organic fertilizer as a protection of sweet basil against P.aeruginosa disease. The organic fertilizer was prepared by fermentation for 21 days. The pH of the fermented fertilizer and the pH of the soil after application of fertilizer were measured. The sweet basil was grown for 6 weeks and followed by the introduction of P.aeruginosa to infect the plant leaves. The infected plants were monitored for 5 days for post-infection symptoms. P.aeruginosa proliferation on the leaves after infection were checked by performing colony count on LB agar plate. The infected plant leaves with applied of fertilizer were observed to survive at day 4. Meanwhile, the infected plant leaves without fertilizer demonstrated infection symptoms on day 3 and fell off on the next day. Overall, the application of fertilizer was found to enhance the defense system of the plant model against P.aeruginosa.