Phytochemical Study of Strobilanthes Crispa Leaves and Its Antioxidant and Antibacterial Properties

Tang, Huan Ting (2024) Phytochemical Study of Strobilanthes Crispa Leaves and Its Antioxidant and Antibacterial Properties. Final Year Project (Bachelor), Tunku Abdul Rahman University of Management and Technology.

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Abstract

This research project focused on the extraction and characterization of bioactive compounds from S. crispa leaves, particularly highlighting the isolation and characterization of 2,5-di-tert-butylphenol (2,5-DTBP). The extraction process involved maceration with 80% ethanol and liquid-liquid partition, resulting in the successful isolation of the ethyl acetate (EA) extract with a yield of 0.91%. Phytochemical screening revealed the presence of alkaloids, phenols, and flavonoids, indicating the extract’s rich chemical composition. Further characterization using Fourier Transform Infrared (FT-IR) spectroscopy identified diverse functional groups within the EA extract. TLC profiling, FTIR, and Nuclear Magnetic Resonance (NMR) analyses were employed to characterize the isolated compound, 2,5-DTBP. Evaluation of antioxidant activity via the DPPH assay demonstrated significant potency for both EA extract (EC50 = 48.27 ± 3.33 μg/mL) and 2,5- DTBP (EC50 = 35.78 ± 0.78 μg/mL). However, the disc diffusion assay showed no inhibitory effects against Staphylococcus aureus and Escherichia coli at the concentration of 400 μg/mL to 50 μg/mL for EA extract and 200 μg/mL to 25 μg/mL for 2,5-DTBP. This research study facilitates future research on the isolation and identification of pharmacological agents. Understanding the therapeutic potential of these compounds, especially 2,5-DTBP with strong antioxidant properties, could pave the way for developing treatments to combat oxidative stress. Overall, this research highlights S. crispa leaves as a promising source of antioxidants for future investigation.