Metabolite Profiling of Potassium Oxonate-Induced Hyperuricemic Rat Serum Using Metabolomic Approach

Lim, Su Xuen (2025) Metabolite Profiling of Potassium Oxonate-Induced Hyperuricemic Rat Serum Using Metabolomic Approach. Final Year Project (Bachelor), Tunku Abdul Rahman University of Management and Technology.

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Abstract

Gout is a painful form of arthritis that is faced by many globally, which is primarily caused by hyperuricemia (i.e. the condition of elevated uric acid levels in the blood) that subsequently resulted in the deposition of crystals at joint areas. Chrysanthemum morifolium has been the study of interest of researchers worldwide for its various bioactivities including anti-hyperuricemic effect, where luteolin and eriodictyol-7-O-glucoside were discovered to be the contributors of such an effect. In this study, metabolite profiling was performed on potassium oxonate-induced hyperuricemic rat serum using metabolomic approaches, which was then coupled with chemometric techniques to investigate its correlation with the serum uric acid (SUA) level of the rats. Anti-hyperuricemic study was conducted on Sprague-Dawley rat models, which are blank (B), positive (P) and negative (N) controls, treatment of luteolin (L), eriodictyol-7-O-glucoside (E), and the combination (C) of both compounds in a 1:10.6 ratio, respectively. The compounds were administered daily for 7 days via oral gavage. The rats were sacrificed on day 7, and blood serum was collected for proton nuclear magnetic resonance (¹H NMR) analysis and serum uric acid (SUA) assay. From the multivariate analyses of both data, clustering was observed between group B, P and E, revealing the potential of eriodictyol-7-O-glucoside in lowering SUA level. Significant elevation (p < 0.05) of alanine was also noted in the positive controls, denoting its potential to be a metabolic biomarker for the positive SUA-lowering effect. The study concluded that eriodictyol-7-O-glucoside displayed prospective anti-hyperuricemic activity, whereas luteolin showed minimal effect and possible antagonistic activity with eriodictyol-7-O-glucoside at lowering SUA level. Future studies may delve into the SUA lowering mechanism of eriodictyol-7-O-glucoside, so as to examine the necessity of structural alterations or its incorporation into commercial drugs for improved potency or reduced side effects. Keywords: Gout; Anti-hyperuricemic; NMR; SUA; Metabolomics.